Zoya Ahmed

Zoya Ahmed

Biography

Zoya Ahmed is a Senior at Xavier University of Louisiana, studying Public Health Sciences with a minor in biology, chemistry, and history, planning to pursue her PhD in Women’s Health. As a New Orleans native, connecting with her community and interning at the Ochsner-Xavier Institute for Health Equity Research (OXIHER) are what defined her passion for managing insulin resistance-related diseases through traditional and holistic care. She strengthened her passion for bridging the gap between clinical medicine and community-level public health interventions while working on the STRIVE study with Tulane’s SPHTM. This summer, Zoya is working with Dr. Benoit Desbiolles in the Shalek Lab, focusing on fabricating freestanding micro-nanodevices to transport through biological tissues. In the future, Zoya aims to investigate how epigenetics, psychosocial stressors, and dietary inflammatory markers contribute to metabolic dysfunction within high-risk populations and how these factors may lead to disproportionate health diagnoses and outcomes.

Freestanding Micro-Nano Devices for Studying 3D Tissue
Zoya Ahmed¹, Benoit Desbiolles^2-5, DK Alakwe^2-5, and Alex Shalek^2-5
1Department of Public Health Sciences, Xavier University of Louisiana
²Department of Chemistry, Massachusetts Institute of Technology
³Institute of Medical Engineering and Science, Massachusetts Institute of Technology
 ⁴Broad Institute of MIT and Harvard University
⁵Ragon Institute of Massachusetts General Brigham, MIT, and Harvard University

Studying cells in their native three-dimensional (3D) environment is essential for understanding cellular function. Cells rely on complex interactions with neighboring cells and the extracellular matrix to regulate fundamental biological processes, behaviors that are heavily shaped by the mechanical and chemical cues of their native 3D environment. However, most existing technologies are limited to two-dimensional (2D) models, highlighting the need for tools that enable cellular interrogation within intact 3D tissues. To address this need, we developed freestanding micro/nanodevices capable of penetrating and distributing throughout 3D tissue to wirelessly probe and modulate cellular characteristics in their native microenvironment need to be developed. This project evaluated centrifugation and electrophoresis as actuation modalities for delivering freestanding micro/nanodevices into tissue. First, we developed a theoretical model to estimate the forces generated by each modality and compare them with the force required to penetrate the extracellular matrix. Next, we developed a scalable micro/nanofabrication process to manufacture the devices. Finally, we characterized device distribution within agarose phantom tissue using optical microscopy to validate our theoretical predictions. Identifying the optimal delivery modality will enable device distribution throughout intact 3D tissues, advancing the study of cellular characteristics within their native architecture.